Enzyme Lab #2: The Effect of Temperature on Enzyme Activity

SWBIC Educational Resources  >> Activities & Materials  >> Enzyme Unit Plan  >> LAB 2


After preparing and filtering a red potato, (as previously described) pour approximately 20 ml of the liquid into each of five test tubes, labeled A-E. The five test tubes should be placed in water baths at or near the following temperatures:

  1. 5 degrees Celsius
  2. 20 degrees Celsius
  3. 37 degrees Celsius
  4. 65 degrees Celsius
  5. 98 degrees Celsius

Now, set up five more test tubes, labeled A-E, in a test tube rack; to each, add 20 ml H2O2.

After each of the first five test tubes — containing the enzyme — have sat at their temperatures for at least ten minutes, dump their contents into the second set of test tubes. Make sure you dump the enzyme from A into the other A, B into B, C into C, etc.

After a minute or two, use a ruler to measure the height of the foam produced. Record these amounts.


  1. At which temperature was the enzyme most active? (Which one produced the most foam?)
  2. At which temperature was the enzyme least active?
  3. Construct a line or bar graph, temperature (x-axis) vs. foam height (y-axis).
  4. What does your graph say about enzyme activity? Be specific as to temperatures.


Are enzymes equally effective at all temperatures? Explain your answer.

Developed for SWBIC by John Palmer, Gadsden High School.