Module 2: Storage, Retrieval and Manipulation of DNA Information (General Themes)
How to deposit and retrieve DNA sequences?
|There are different ports of entry to access DNA information (See Module 3 for a list and more
detailed discussion.) already stored in various databases. Let us assume that a research group has sequenced a new segment of DNA from
an unidentified clone or from a cloned gene. Then, the next step is to check if there is any DNA sequence "out there" that resembles the
newly sequenced DNA. If there is any sequence similarity to a database sequence(s), it would be interesting to find out the percentage
of similarity (or homology) between the 2 sequences. The research group will most likely submit the test sequence to a database so that
other researchers can retrieve and manipulate the new data as part of the database.
Since DNA sequences are being submitted by research laboratories from all over the world, there is a lack of uniformity in the
submission & stored data formats and in the accessing tools available to different laboratories. Hence, one has to be aware of the
submission format requirements of different databases and their output formats when accessing results from database search queries. We
will cover these topics in much more detail as we get into later modules.